Assessment of Aerobic and Respiratory Growth in the Lactobacillus casei Group
نویسندگان
چکیده
One hundred eighty four strains belonging to the species Lactobacillus casei, L. paracasei and L. rhamnosus were screened for their ability to grow under aerobic conditions, in media containing heme and menaquinone and/or compounds generating reactive oxygen species (ROS), in order to identify respiratory and oxygen-tolerant phenotypes. Most strains were able to cope with aerobic conditions and for many strains aerobic growth and heme or heme/menaquinone supplementation increased biomass production compared to anaerobic cultivation. Only four L. casei strains showed a catalase-like activity under anaerobic, aerobic and respiratory conditions and were able to survive in presence of H2O2 (1 mM). Almost all L. casei and L. paracasei strains tolerated menadione (0.2 mM) and most tolerated pyrogallol (50 mM), while L. rhamnosus was usually resistant only to the latter compound. This is the first study in which an extensive screening of oxygen and oxidative stress tolerance of members of the L. casei group has been carried out. Results allowed the selection of strains showing the typical traits of aerobic and respiratory metabolism (increased pH and biomass under aerobic or respiratory conditions) and unique oxidative stress response properties. Aerobic growth and respiration may confer technological and physiological advantages in the L. casei group and oxygen-tolerant phenotypes could be exploited in several food industry applications.
منابع مشابه
Effects of Lactobacillus casei probiotics on growth performance, blood parameters and intestinal microbial flora in juvenile goldfish (Carassius auratus)
Lactobacillus casei probiotic is one of the probiotics used in aquaculture industry. The aim of this study was to evaluate the effect of Lactobacillus casei probiotic on the growth performance, blood parameters, and intestinal microbial flora in goldfish (Carassius auratus) for 60 days. In this study, 180 fish with an average weight of 10.11± 0.16 g were randomly distributed into two treatments...
متن کاملInvestigation of Factors Affecting Aerobic and Respiratory Growth in the Oxygen-Tolerant Strain Lactobacillus casei N87
Aerobic and respiratory cultivations provide benefits for some lactic acid bacteria (LAB). Growth, metabolites, enzymatic activities (lactate dehydrogenase; pyruvate and NADH oxidases, NADH peroxidase; catalase), antioxidant capability and stress tolerance of Lactobacillus casei N87 were evaluated in anaerobic, aerobic and respiratory (aerobiosis with heme and menaquinone supplementation) batch...
متن کاملبررسی اثر رشد و تکثیر تعدادی از باکتریهای لاکتیک روی تحول میکروبی گوشت چرخ کرده گاو در شرایط صنعتی
The effects of growth of two strains of lactic acid bacteria on microbial flora of ground beef after packaging in air permeable packs were studied under workshop conditions. The strains isolated from dairy products were added to ground beef at a density of 104 CFU/g after identification and antibiosis test against indicator bacteria (Escherichia coli and Staphylococcus aureus). The two strains ...
متن کاملبررسی اثر رشد و تکثیر تعدادی از باکتریهای لاکتیک روی تحول میکروبی گوشت چرخ کرده گاو در شرایط صنعتی
The effects of growth of two strains of lactic acid bacteria on microbial flora of ground beef after packaging in air permeable packs were studied under workshop conditions. The strains isolated from dairy products were added to ground beef at a density of 104 CFU/g after identification and antibiosis test against indicator bacteria (Escherichia coli and Staphylococcus aureus). The two strains ...
متن کاملEvaluation of Cell Growth and Substrate Consumption Kinetic of Five Different Lactobacilli in a Submerged Batch Whey Culture for Lactic Acid Production
Cell growth and lactose consumption profile of five Lactobacillus Strains: bulgaricus, casei, lactis, delbrueckii and fermentum has been investigated. Experiments of cell growth and substrate utilization were conducted in batch submerged culture of whey with added lactose and some other growth factors. Fitness assessment of experimental data on the cell growth and lactose consumption by Monod k...
متن کامل